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Personal Protective Equipment (PPE):

  • Use appropriate personal protective equipment during all times in the Laboratory
  • All individuals working in the chemical laboratory must wear aprons and shoes all the time
  • Use safety goggles while handling any chemicals in the laboratory
  • Use nitrile gloves and mask while handling solvents and solid chemicals
  • Use acid/alkali gloves while handling acid and alkali
  • Use heat resistant gloves while heating/boiling or handling hot glassware

General Laboratory Operations:

  • No equipment should be kept next to the door as this prevents complete opening of the door.
  • All unwanted chemicals and glassware should be removed from work table immediately
  • Work table and floor should be cleaned after each stage of an experiment and after spillage.
  • Broken glass pieces should be removed and swept up immediately
  • All the reagents (acid, solvent etc.) should be labelled and kept properly at their place.
  • Avoid naked wires running across the laboratory floor.
  • Each equipment should be placed at its specified place
  • The first aid box should be placed at designated place at easy access.
  • The samples for analysis should be kept in their designated places.

Handling of Chemicals and Solvents:

  • All chemicals should be handled with care.
  • Spills on hand or other parts of body, should be immediately cleaned/washed thoroughly.
  • The acid spill should be continuously washed with cold running water at least till the burning sensation ceases. Immediately consult a physician.
  • Transferring or weighing of chemicals should be done with a spatula.
  • Do not breath chemical vapours or gases.
  • Do not attempt to smell during the course of reaction.
  • Do not pipette by mouth, instead use appropriate rubber bulbs.
  • Do not store dangerous chemicals on high shelves.
  • Handle organic solvents away from the open flames, if any.
  • Heating of acid should be carried out in the fume hood/cabinet only.
  • Dilution of acid should be done by carefully pouring acid in water and not vice-versa.
  • All solvents should be regarded as flammable.
  • Turn off all naked flames while using solvents.
  • Used solvents should be disposed carefully.

Handling glassware and apparatus:

  • Examine all glassware for defects before any experiment; do not use cracked or badly chipped glassware.
  • The damaged glassware should be carefully discarded and replaced appropriately.
  • Always remove chemicals from glassware before washing.
  • Handle the reagent bottles carefully; use appropriate trays to carry from one place to another.
  • Never leaves any reagent bottles, measuring cylinders etc. standing on the edge of the work table where it can easily be knocked over.
  • Do not setup apparatus near the edge of the work table.

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